Table 2.

Summary of serum PrV-specific IgG levels of the animals immunized with replication-defective adenovirus expressing glycoproteins, gB, gC, and gD.

^aC57BL/6 (n = 7) mice were immunized i.m. with rAd expressing gB (rAd-gB), gC (rAd-gC), and gD (rAd-gD) as described in Materials and Methods. Control mice were given replication-incompetent adenovirus expressing LacZ gene (rAd-LacZ). ^bSeven days after each immunization, PrV-specific IgG levels in sera were determined by conventional ELISA. The values represent the average ± SD from seven mice per group. ^cSeven days after the final immunization, The levels of PrV-specific IgG isotypes (IgG2a and IgG1) in sera were determined by ELISA. The values represent the average ± SD from seven mice per group. ^dSignificantly different from values obtained for rAd-gB-immunized group with rAd-gC-immunized group (P < 0.01). ^eSignificantly different from values obtained for rAd-gB-immunized group with rAd-gD-immunized group (P < 0.01). ^fSignificantly different from values obtained for rAd-gC-immunized group with rAd-gD-immunized group (P < 0.05).