Figure 3.

Immunoblot analysis of differentially secreted proteins in conditioned media and whole cell lysates. The conditioned media and whole cell lysates were prepared from BEAS-2B, 1799, 1198 and 1170-I cells. Proteins were separated on 10% or 12.5% SDS-PAGE gels, and visualized using ECL method after incubation with the primary antibodies against GAPDH, PGP9.5, PRX6, SPARC, TCTP, TIMP-2, and TPI. Lamin B proteins were used as an internal control to correct protein loading errors. Data are a representative of three or four independent experiments with similar results.