Figure 4.

Immunoblot analysis of differentially secreted proteins in lung cancer tissues and adjacent normal lung tissues. Matched normal and tumor tissues obtained from 11 lung cancer patients [6 SCCs and 5 adenocarcinomas] were used. Proteins (20 to 50 μg) were separated on 10% or 12.5% SDS-PAGE gels and then transferred to nitrocellulose membranes. The membranes were incubated with the primary antibodies against β-actin, PGP9.5, PRX6, TCTP, and TPI. Proteins were visualized by ECL method. β-actin proteins were used as an internal control to correct protein loading errors. Data represent one of three independent experiments with similar results.