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Inhibitory effect of PEP-1-PTEN on LPS-induced iNOS and COX-2 mRNA levels in Raw 264.7 cells. Raw 264.7 cells were pretreated with the PEP-1-PTEN for 1 h before incubation with LPS (1 μg/ml) for 12 h. Total RNA was extracted. iNOS and COX-2 mRNA was analyzed by RT-PCR using specific primers.
