Figure 4.

Involvement of JNK in DXR-induced PKCδ promoter activation. (A) NIH 3T3 cells grown in 12-well plates were co-transfected with 0.5 µg of mPKCδ-Luc(-1192/+10) and 5 ng of pRL-null vector, along with or without 0.2 µg expression plasmid for JNK1 or DN-JNK1, as indicated. At 24 h post-transfection, the cells were treated with 1 µg/ml DXR for 8 h, as indicated. (B) NIH 3T3 cells grown in 12-well plates were co-transfected with 0.5 µg of PKCδ-Luc(-1192/+10) and 5 ng of pRL-null vector. After 24 h, the cells were pretreated with the indicated concentrations of SP600125 for 30 min, followed by addition of 1 µg/ml DXR for 8 h. Firefly luciferase activity was normalized to Renilla luciferase activity. The data shown represent the mean ± S.D. (error bars) of three independent experiments performed in triplicate.