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. 2008 Dec 31;40(6):699–708. doi: 10.3858/emm.2008.40.6.699

Figure 5.

Figure 5

Role of c-Jun/ATF2 binding motif in DXR-induced PKCδ promoter activation. (A) Nucleotide sequence of the 30 bp (-846 to -816) in the 5'-flanking region of the mouse PKCδ gene. The predicted binding site for c-Jun or ATF2 is marked as a solid or dotted box, respectively. Consensus c-Jun/ATF2 heterodimer binding site is marked as a grey box. (B) NIH 3T3 cells were transfected with wild-type mPKCδ-Luc(-1192/+10), mPKCδ-Luc(-1192/+10)mtJunATF2, or mPKCδ-Luc(-683/+10), along with 5 ng of pRL-null vector. The putative c-Jun/ATF2 binding motif is shown schematically. Arrowheads indicate the sites of point mutations. After 24 h post-transfection, the cells were either untreated or treated with 1 µg/ml DXR for 8 h. Firefly luciferase activity was normalized to the Renilla luciferase activity. The data shown represent the mean ± S.D. (error bars) of three independent experiments performed in triplicate.