Figure 3.

The critical role of PKCµ on cell viability under hypertonic condition. Increase of hypertonicity-induced cell death due to the treatment of PKCµ inhibitor (A) or PKCµ Si duplex (B) NIH-3T3 cells were treated with Gö6976 or transiently transfected with PKCµ RNAi duplexes prior to exposure to hypertonic conditions for 12 h. Cell viability was assessed via MTT assay. Values are expressed as the means ± standard deviation of triplicate determinations. Cell lysates were also resolved via SDS-PAGE and blotted with anti-HSP70 and anti-caspase-3 antibodies.