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Increased tyrosine phosphorylation of 53 kDa by K15 expression. A total of 2 × 106 Bjab cells were stimulated with either PBS or OKT8 (anti-CD8) antibody for 5 min and the cell extracts were subjected to a western blot with antiphospho tyrosine immunoblot. At the bottom, the cell surface expression of CD8 chimera proteins were measured by anti CD8-PE antibody.
