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. Author manuscript; available in PMC: 2009 Dec 25.
Published in final edited form as: J Med Chem. 2008 Dec 25;51(24):8158–8162. doi: 10.1021/jm801254r

Figure 3.

Figure 3

Inhibition of caspase-3/-7 activity by XIAP and antagonism of Smac mimetics to XIAP to recover the activity of caspase-3/-7 in a cell-free functional assay. (A). Caspase-3/-7 was activated by addition of cytochrome c and dATP into cell lysates and recombinant XIAP BIR3 protein at 0.5 μM completely inhibited caspase activation. Compound 6 dose-dependently recovered the caspase-3/-7 activity. (B). Dose-dependent recovery of caspase-3/-7 activity by 1, 4 and 6 to the maximum activation. Caspase-3/-7 activity at 30 minute time point was used.