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Effect of aging on BMSC proliferation. BMSCs from 3-, 6-, 12-, 18-, and 24-mo-old mice were plated in triplicate in 96-well plates at a density of 1 × 104 cells/cm2, and cell proliferation was assayed using a Cell Growth Determination kit for 7 consecutive days. These experiments were repeated in triplicate in two separate experiments (*p < 0.01 compared with 3 mo).
