. Author manuscript; available in PMC: 2009 May 8.

Published in final edited form as: Clin Exp Metastasis. 2008 Jul 23;25(7):799–810. doi: 10.1007/s10585-008-9193-z

Table 1.

Summary of immunofluorescence analysis of surgical brain metastasis specimens from breast cancer patients

Sample #	Age^a	ER status^b	Histologic classification^b	TNM classification^b	Proliferative index (%)^c	Cell death^d	Cytokeratin content^e	Glial content^f
1	36	-	Ductal	T₃N₁	53	+	+++	+++ (islands)
3	43	-	Ductal	T₂N₀M₀	50	+++	+++	++ (ribbons)
4	57	+	Ductal-lobular	T₄N₀M₁	39	++	++	++ (ribbons)
5	36	+	Ductal	T₂N_1aM₁	56	+	+	+ (at margins)
7	64	-	Ductal	T₁N₁	59	+	+++	+ (at margins)
11	66	-	Ductal	T₂	42	+++	+++	+++ (islands)
13^g	68	-	Ductal	T₁N₀M₀	32	+	+++	-
14^g	68	-	Ductal	T₁N₀M₀	41	+++	+++	+++ (islands)
15	50	-	Ductal with DCIS	T₂N₀M₀	61	+++	++	+
18	60	+	Ductal	T₁N₀M₀	57	No data	++++	-
21	60	-	Ductal	T₂N₀	28	+++	+++	+++ (islands)
22	54	-	Lobular	T_1cN₂	9	++	+++	++++ (ribbons)
23	58	+	Lobular	T₂N₂	24	+	++++	++ (ribbons)
24	58	-	Inflammatory	T_1cN_x	50	+++	++ (discrete clusters)	++++
25^h	61	-	Ductal	T₂N₁M₀	48	+++	+++	+++ (islands)
26^h	61	-	Ductal	T₂N₁M₀	48	+++	+++	+++ (islands)

Age of patient at time of diagnosis of brain metastasis

Classification of the primary tumor (ER = estrogen receptor)

Percentage of Ki67-positive cells among Cytokeratin-positive metastatic cells (>150 cell nuclei counted per sample)

Amount of Cleaved (Active)-Caspase-3 staining in metastatic brain tumors, includes areas of necrosis (scale of 1 to 3⁺, ⁺⁺⁺ indicates extensive necrosis)

Amount of Cytokeratin-positive staining in metastatic brain tumors (anti-Cytokeratin MNF116, scale of 1 to 4⁺)

Amount of GFAP-positive astrocyte staining in metastatic brain tumors (scale of 0 to 4⁺)

Individual brain metastases removed from the same patient