FIGURE 3.

Virion incorporation of the Nef7.A3G fusion protein. 293T cells were transfected with HIV.env^–.nef^– and WT Nef, Nef7, Nef7.A3G, or A3G expression plasmids with pcDNA3 included as a control. Progeny viruses were collected and subjected to Western blot (WB) analysis along with whole cell lysate using an antibody against an HA epitope that was added to the C terminus of all Nefs, Nef7.A3G, and A3G and antibodies against HIV-1 p24 or β-actin as loading controls (A) followed by quantitation of the protein level in virions from three independent experiments, as stated above (B).