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Ligand concentration-dependent fluorescence emission intensity of wild type and variant sLDLR. Fourμg of a given sLDLR was incubated with increasing amounts of AEDANS-Trp-null apoE3-NT·DMPC. Open circles, WT sLDLR; filled circles, LA5@2 sLDLR; open squares, LA2@5 sLDLR; and filled squares, LA5⇔2 sLDLR. Fluorescence emission intensity values reported were obtained after subtraction of fluorescence emission intensity of ligand alone at each concentration.
