TABLE 2.
Distribution of the radiolabeled glucose after incubation of permeabilized cells or membrane extracts with UDP-[14C]glucose
| Cell fractions | Permeabilized cellsa | Membrane extractsa |
|---|---|---|
| nmol glucose incorporated/h/μg protein | nmol glucose incorporated/h/μg protein | |
| Alkali-soluble fraction | 0.28 ± 0.02 | 0.09 ± 0.03 |
| Alkali-insoluble (AI) | 1.17 ± 0.11 | 0.77 ± 0.03 |
| AI β-(1,3)-glucan | 0.75 ± 0.16 | 0.63 ± 0.04 |
| AI β-(1,6)-glucan (23.6 min peak) | 0.24 ± 0.04 | 0 |
a
Values were obtained from an average of five experiments, as determined by using a liquid scintillation counter (incubations were performed with a reaction mixture containing UDP-glucose (0.5 mm containing UDP-[14C]glucose), Tris-HCl buffer (50 mm, pH 7.0), ATP (0.2 mm), GTPγ S (20 μm), MgCl2 (0.2 mm), EDTA (10 mm), and N-acetylglucosamine (20 mm) for 60 min at room temperature in 67 μl.