FIGURE 1.

IFN-γ induces ASK1 phosphorylation and degradation in EC. a and b, IFN-γ induces ASK1 degradation in WT but not in SOCS-KO EC. WT (a) and SOCS1-KO (b) MLEC were treated with TNF, IFN-β, or IFN-γ at 10 ng/ml for 16 h. Cell lysates were subjected to Western blot with antibodies against ASK1, SOCS1, TRAF1, and tubulin. c, IFN-γ induces ASK1 phosphorylation at Tyr-718. WT and SOCS1-KO EC were serum-starved for 12 h followed by treatment with TNF, IFN-β, or IFN-γ (10 ng/ml for 15 min). Phospho- and total ASK1 were determined by Western blot with anti-Tyr(P)-718 (pY718) and anti-ASK1 antibody, respectively.