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. 2009 May 15;284(20):13481–13488. doi: 10.1074/jbc.M809740200

FIGURE 2.

FIGURE 2.

JAK2 associates with ASK1 and mediates IFN-γ-induced ASK1 phosphorylation. a, HAEC were untreated or pretreated with AG490 (AG) (10 μm) for 60 min followed by IFN-γ (10 ng/ml) for 15 min. Phosphorylation of JAK2 and ASK1 was determined by Western blot with phospho-specific antibodies. Ctrl, control. b, JAK2 associates with ASK1. HAEC were treated with IFN-γ for the indicated times. Phospho- and total JAK2, STAT1, and ASK1 were determined by Western blot with respective antibodies. ASK1-JAK2 complex was determined by immunoprecipitation (IP) assay with anti-JAK2 followed by Western blot with anti-ASK1. c, JAK2-ASK1 complex is stabilized by proteasomal inhibitor MG132. HAEC were pretreated with MG312 for 60 min followed by IFN-γ treatment for 30 min. JAK2-ASK1 complex was determined as in b.