FIGURE 2.

Proton concentration mediates binding of TLF to target membranes. Liposome binding assays were performed with soy bean azolectin liposomes in 50 mm Tris-maleate, pH 5.0, or 50 mm Tris, pH 6.8. Supernatant (S), 1 m KCl wash (W), and pellet (P) fractions were analyzed by non-reducing SDS-PAGE. The entire TLF protein component appears in the pellet fraction at pH 5.0 but not pH 6.8. The dimeric and tetrameric forms of Hpr (indicated by Hpr dimer and Hpr tetramer) and the full-length and truncated apoL-1 bands (both indicated by apoL-1) are indicated.