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NMR characterization of NS5A-D2 (JFH1). A, assigned 1H,15N HSQC spectrum of domain 2 of NS5A (JFH1). The smaller insert shows the spectrum region corresponding to the two tryptophan side chains. B, CSI analysis of NS5A-D2. NS5A-D2 13Cα, 13Cβ, and 13CO chemical shifts were analyzed using CSI software (54). The consensus CSI is zero along the complete sequence and therefore is not shown.
