TABLE 3.

Comparison of in vivo and in vitro K_D measurements of Cdc42-effector interactions The Cdc42-CRIB, -N-WASP, and -IRSp53 interactions were measured in vivo using FCCS, and data are taken from the plots presented in Fig. 5. Cdc42-CRIB and WASP interactions were in vitro, and data are taken from Owen et al. (24). Cdc42-IRSp53 binding was measured as described under “Materials and Methods,” and K_D is an average ± S.D. (n = 6). N-WASP and WASP are isoforms with almost identical Cdc42 binding sequences.

Proteins	CRIB in vivo	CRIB in vitro	N-WASP in vivo	WASP in vitro	IRSp53 in vivo	IRSp53 in vitro
KD	250 ± 25	620 ± 138	27 ± 3	55 ± 6	391 ± 33	880 ± 86
Source	This study	Ref. 24	This study	Ref. 24	This study	This study