FIGURE 3.

The analysis of V_H5-51/D3-9/J_H4 recombinations derived from different cancer types. These V_Hγ D_γ J_Hγ sequences showed almost identical mutation targets over the V segment. Somatic mutations were identified by comparison with the published germline V_H5-51 gene in NCBI. The RGYW/WRCY motif was underlined. The first 25 nucleotides were excluded from the analysis because they were encoded by the primers. The clone name appears in four characters: the first character represents the tissue origin (B for breast cancer, L for lung cancer, C for colon cancer, and O for oral cancer); the second represents the immunoglobulin isotype (G for IgG); the third represents the patient number, and the last represents the clone number.