TABLE 2.
Assignment of the likely matching germline variable region genes to the VHDJH recombinants from different cancer types and analysis of the V gene somatic mutation rate
| Cases | No. of clones | VHγDJHγ (no. of clone) | Mutation rate | No. of clones | VHμDJHμ (no. of clone) | Mutation rate |
|---|---|---|---|---|---|---|
| Breast cancer (case 1) | 5 | VH5-51/D3-9/JH4(3) | 15.6% (3) | 3 | VH4-30-2/D3-22/JH4(1) | 3.3% (1) |
| VH7-4-1/D2-8JH4(1) | 1% (1) | VH3-33/D3-10/JH4(1) | 2.2% (1) | |||
| VH3-9/D3-9/JH3(1) | stopa (1) | VH3-33/D5-12/JH5(1) | 0 (1) | |||
| Breast cancer (case 2) | 5 | VH5-51/D3-9/JH4(4) | 15.6% (2); 17.5% (1) | 3 | VH4-30-2/D3-22/JH4(1) | 0 (1) |
| stop (1) | VH3-33/D5-12/JH5(1) | 4.4% (1) | ||||
| VH1-16/NI/JH4(1) | stop (1) | VH3-15/D3-10/JH4(1) | 1.1% (1) | |||
| Breast cancer (case 3) | 2 | VH5-51/D3-9/JH4(1) | 15.6% (1) | 4 | VH4-30-2/D3-22/JH4(1) | 3.3% (1) |
| VH3-33/D5-12/JH5(1) | 0 (1) | |||||
| VH3-30/D6-19/JH4(1) | 13.5% (1) | VH4-15/D6-13/JH4(1) | 10% (1) | |||
| VH4-61/D6-13/JH4(1) | 0 (1) | |||||
| Lung cancer | 6 | VH5-51/D3-9/JH4(2) | 15.6% (2) | 2 | VH6-1/D6-13/JH4(1) | 3.3% (1) |
| VH3-30/D6-9/JH4(2) | 12.5% (2) | VH6-1/D1-7/JH6(1) | 2.1% (1) | |||
| VH1-3/D5-12/JH4(2) | 28.1% (2) | |||||
| Colon cancer (case 1) | 4 | VH5-51/D3-9/JH4(4) | 15.6% (4) | 5 | VH3-15/D3-10/JH4(4) | 3.5% (4) |
| VH4-30-2/D3-22/JH4(1) | stop (1) | |||||
| Colon cancer (case 2) | 7 | VH5-51/D3-9/JH4(4) | 15.6% (3), 17.5% (1) | ndb | nd | nd |
| VH3-30/D6-9/JH4(1) | 13.5% (1) | |||||
| VH1-69/D6-15/JH4(1) | stop (1) | |||||
| VH7-4-1/D3-10/JH4(1) | 4% (1) | |||||
| Oral cancer (case 1) | 3 | VH5-51/D3-9/JH4(2) | 15.6% (1), stop (1) stop (1) | 9 | VH6-1/D6-13/JH4(4) | 0 (4) |
| VH1-2/D2-8/JH4(1) | VH6-1/D1-7/JH6(3) | 7.6% (3) | ||||
| VH6-1/D2-2/JH4(2) | 2.5% (2) | |||||
| Oral cancer (case 2) | 9 | VH3-33/D6-19/JH5(6) | 11.5% (5); | nd | nd | nd |
| VH3-23/D1-26/JH4(3) | 16.3% (1) 7.3% (3) | |||||
| HT-29 | 7 | VH5-51/D2-2/JH4(1) | 15.% (1) | 6 | VH6-1/D6-13/JH4(6) | 3.3% (4), 1% (1), 7.6% (1) |
| VH3-33/D3-10/JH5(1) | 5% (1) | |||||
| VH1-8/D5-18/JH5(1) | 18% (1) | |||||
| VH1-69/D1-26/JH5(1) | stop (1) | |||||
| VH3-23/D2-15/JH4(1) | 7.1% (1) | |||||
| VH3-30/D6-13/JH4(1) | 9.2% (1) | |||||
| V1-3/D3-10/JH5(5) | 8.3% (1) | |||||
| HelaS3 | 6 | VH1-8/D1-14/JH4(1) | 8.1% (1) | 3 | VH3-30-3/D6-19/JH5(3) | 1.5% (3) |
| VH1-8/D2-2/JH4(1) | 6.2% (1) | |||||
| VH4-59/D3-10/JH4(1) | 10.4% (1) | |||||
| VH4-59/D3-22/JH4(1) | 7.5% (1) | |||||
| VH3-30/N1/JH5(1) | stop (1) | |||||
| VH3-7/D3-10/JH4(1) | 9.1% (1) | |||||
| PBL | 3 | VH3-23/D3-10/JH4(1) | 4.8% (1) | 4 | VH3-21/D1-26/JH4(1) | 0 (1) |
| VH4-59/D1-20/JH4(1) | 4.8% (1) | |||||
| (case 1) | VH1-69/D5-5/JH4(1) | 11.5% (1) | VH4-39/D3-10/JH5(1) | 2.9% (1) | ||
| VH1-8/D2-2/JH5(1) | 12.5% (1) | VH3-23/D6-19/JH5(1) | 14.4% (1) | |||
| PBL | 3 | VH4-59/D3-3/JH4(1) | 20% (1) | nd | nd | nd |
| VH4-59/D4-17/JH4(1) | 17.9% (1) | |||||
| (case 2) | VH3-43/NI/JH5 (1) | 3.1% (1) |
a
Stop, non-functional VHDJH sequences
b
nd, not done