TABLE 3.
Seven sets of predominant VHDJH recombinants found in different cancer types The clone name appears in four letters as follows: the first represents the tissue of origin (B for breast cancer, L for lung cancer, C for colon cancer, O for oral cancer, and H for HT-29 cell line); the second represents immunoglobulin isotype (G for IgG, and M for IgM); the third represents the patient number; and the last number in brackets represents the number of clones in this set of VHDJH rearrangements.
| No. of sets | Clone | V gene name | V region | N1 | P | D region | N1 | P | J region | J gene name | D gene name |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | BG1 (3) | IGHV5-51*01 | tgtgcgaga | tgggatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | ||
| BG2 (4) | IGHV5-51*01 | tgtgcgaga | tgggatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | |||
| BG3 (1) | IGHV5-51*01 | tgtgcgaga | tgggatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | |||
| CG1 (4) | IGHV5-51*01 | tgtgcgaga | tgggatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | |||
| CG2 (4) | IGHV5-51*01 | tgtgcgaga | tgggatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | |||
| LG1 (1) | IGHV5-51*01 | tgtgcgaga | tggaatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | |||
| OG1 (2) | IGHV5-51*01 | tgtgcgaga. | tgggatg | ...........ttatgattggattttata.. | cagc | .......tgactactgg | IGHJ4*02 | IGHD3-9*01 | |||
| 2 | BG3 (1) | IGHV3-30*01 | tgtgccagaga | ......aacaatggctg.... | cctc | ....ctttgacaattgg | IGHJ4*03 | IGHD6-19*01 | |||
| LG1 (2) | IGHV3-30*01 | tgtgccagaga | ......aacaatggctg.... | cctc | ....ctttgacaattgg | IGHJ4*03 | IGHD6-19*01 | ||||
| CG2 (1) | IGHV3-30*01 | tgtgccagaga | ......aacaatggctg.... | cctc | ....ctttgacaattgg | IGHJ4*02 | IGHD6-19*01 | ||||
| 3 | HM (3) | IGHV6-1*01 | tgtgcaagaga | at | .....tagcagcagct..... | .....tttgactactgg | IGHJ4*03 | IGHD6-13*01 | |||
| OM1 (2) | IGHV6-1*01 | tgtgcaagaga | at | .....tagcagcagct..... | .....tttgactactgg | IGHJ4*02 | IGHD6-13*01 | ||||
| LM (1) | IGHV6-1*01 | tgtgcaagaga | at | .....tagcagcagct..... | .....tttgactactgg | IGHJ4*02 | IGHD6-13*01 | ||||
| OM2 (2) | IGHV6-1*01 | tgtgcaagaga | at | .....tagcagcagct..... | .....tttgactactgg | IGHJ4*02 | IGHD6-13*01 | ||||
| 4 | BM1 (1) | IGHV4-30-2*01 | tgtgccgg... | cc | ..............gaagtggttattact | c | .cccctttgactactgg | IGHJ4*02 | IGHD3-22*01 | ||
| BM2 (1) | IGHV4-30-2*01 | tgtgccgg... | cc | ..............gaagtggttattact | c | .cccctttgactactgg | IGHJ4*02 | IGHD3-22*01 | |||
| BM3 (1) | IGHV4-30-2*01 | tgtgccgg... | cc | ..............gaagtggttattact | c | .cccctttgactactgg | IGHJ4*02 | IGHD3-22*01 | |||
| 5 | BM21 (1) | IGHV3-15*01 | tgtaccacaaa | cctga | ac | gtattactatggttcggggaccga... | accccc | ........gactactgg | IGHJ4*02 | IGHD3-10*01 | |
| CM1 (4) | IGHV3-15*01 | tgtaccacaaa | cctga | ac | gtattactatggttcggggaccga... | accccc | ........gactactgg | IGHJ4*02 | IGHD3-10*01 | ||
| 6 | LM1 (1) | IGHV6-1*02 | tgtgcaagag. | ggcgtacgtgg...... | ccagc | ..........ggacgtctgg | IGHJ6*02 | IGHD1-7*01 | |||
| OM1 (3) | IGHV6-1*01 | tgtgcaagag. | ggcgtacgtgg...... | ccagc | ..........ggacgtctgg | IGHJ6*02 | IGHD1-7*01 | ||||
| 7 | BM2 (1) | IGHV3-33*01 | tgtgcgagaga | .....tggggtggctacgattac | aaggaggtg | ....ctggttcgacccctgg | IGHJ5*02 | IGHD5-12*01 | |||
| BM3 (1) | IGHV3-33*01 | tgtgcgagaga | .....tggggtggctacgattac | aaggaggtg | ....ctggttcgacccctgg | IGHJ5*02 | IGHD5-12*01 |