TABLE 4.
Summary of the mutation frequency in WRCY/RGYW and WA/TW motifs and calculation of the R/S mutation ratios occurring in CDRII and in FWRIII
|
Clone
|
Mutation frequency in RGYW/WRCY
|
Mutation in WA/TW
|
R/S ratio in CDRII and FWRIII
|
||||
|---|---|---|---|---|---|---|---|
| WA | TW | CDRII | FWRIII | ||||
| % | |||||||
| BG1-1 | 62.5 (≥25) | 3 | 0 | 0/1 | 9/4 | ||
| BG1-2 | 16.7 | 3 | 0 | 2/0 (>2.9) | 3/0 | ||
| BG1-3 | 0.0 | 0 | 1 | 0/0 | 0/1 (<1.5) | ||
| BG1-4 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| BG1-5 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| BG2-1 | 62.5 (≥25) | 3 | 1 | 0/1 | 13/6 | ||
| BG2-2 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| BG2-4 | 62.5 (≥25) | 2 | 0 | 0/1 | 11/4 | ||
| BG2-5 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| BG3-2 | 18.2 | 3 | 2 | 4/0 (>2.9) | 4/7 (<1.5) | ||
| CG1-1 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| CG1-2 | 62.5 (≥25) | 2 | 0 | 0/1 | 8/4 | ||
| CG1-3 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| CG1-4 | 62.5 (≥25) | 2 | 0 | 0/1 | 9/4 | ||
| CG2-2 | 0.0 | 0 | 0 | 0/0 | 0/0 | ||
| CG2-3 | 18.2 | 3 | 2 | 4/0 (>2.9) | 4/7 (<1.5) | ||
| CG2-4 | 62.5 (≥25) | 2 | 1 | 0/1 | 9/4 | ||
| LG1-1 | 18.2 | 3 | 2 | 4/0 (>2.9) | 4/7 (<1.5) | ||
| LG1-2 | 18.2 | 3 | 2 | 4/0 (>2.9) | 4/7 (<1.5) | ||
| LG1-3 | 14.3 | 4 | 0 | 2/1 | 8/5 | ||
| LG1-4 | 14.3 | 5 | 1 | 2/1 | 8/5 | ||
| LG1-5 | 62.5 (≥25) | 22 | 0 | 0/1 | 9/4 | ||
| LG1-6 | 62.5 (≥25) | 0 | 2 | 0/1 | 9/4 | ||
| OG1-2 | 62.5 (≥25) | 0 | 2 | 0/1 | 9/4 | ||
| OG2-1 | 9.1 (≥25) | 1 | 2 | 5/2 | 5/3 | ||
| OG2-2 | 23.1 | 0 | 1 | 3/0 (>2.9) | 1/3 (<1.5) | ||
| OG2-3 | 23.1 | 0 | 1 | 3/0 (>2.9) | 1/3 (<1.5) | ||
| OG2-4 | 16.7 | 1 | 2 | 6/1 (>2.9) | 5/2 | ||
| OG2-5 | 16.7 | 1 | 2 | 6/1 (>2.9) | 5/2 | ||
| OG2-6 | 16.7% | 1 | 2 | 6/1 (>2.9) | 5/2 | ||
| OG2-7 | 16.7 | 1 | 2 | 6/1 (>2.9) | 5/2 | ||
| OG2-8 | 16.7 | 1 | 2 | 6/1 (>2.9) | 5/2 | ||
| OG2-9 | 16.7 | 1 | 2 | 6/1 (>2.9) | 5/2 | ||
| SG1-1 | 18.2 | 5 | 3 | 3/1 (>2.9) | 13/5 | ||
| SG1-2 | 45.5 (≥25) | 6 | 3 | 3/0 (>2.9) | 10/6 | ||
| SG1-3 | 10.0 | 0 | 0 | 0/0 | 0/2 (<1.5) | ||
| SG1-6 | 0.0 | 2 | 1 | 1/0 (>2.9) | 7/4 | ||
| HG1-1 | 12.5 | 0 | 1 | 1/1 | 8/0 | ||
| HG1-2 | 7.7 | 0 | 0 | 0/0 | 0/0 | ||
| HG1-3 | 25.0 (≥25) | 3 | 4 | 3/3 | 10/3 | ||
| HG1-4 | 33.3 (≥25) | 5 | 3 | 7/3 | 7/8 (<1.5) | ||
| HG1-5 | 15.4 | 2 | 1 | 2/1 | 4/0 | ||
| HG1-7 | 21.4 | 2 | 2 | 3/0 (>2.9) | 7/3 | ||
| 95 | 35 | ||||||
| 20/43 of Vγ rearrangements showed ≥25% mutation frequency in RGYW/WRCY motifs | 36/43 of Vγ rearrangements showed excess mutations in WA | 8/43 of Vγ rearrangements showed both R/S >2.9 in CDRII and R/S <1.5 in FWRIII | |||||