FIGURE 4.

Co-expression and direct interaction between endogenous Sox10 and ARMCX3. A, RNA was extracted from mouse brain and spinal cord, rat C6 cells, human U87-MG and HeLa cells, and mouse Neuro-2A cells. The RNA was used in multiplex RT-PCR using primers that recognize rat, mouse, and human Sox10, ARMCX3, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, as endogenous control). The left panel presents results obtained from experiments in which reverse transcriptase was included in the RT-PCR, while the right panel presents results obtained when the enzyme was not included in the RT-PCR. B, mitochondria were isolated from OBL21 cells using differential centrifugation. Lysates prepared from the mitochondria were used for Co-IP experiments using anti-Sox10 and anti-ARMCX3 antibodies. Goat IgG and rabbit anti-HA antibody were used as negative controls.