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CNS-specific expression of KCC2a protein in adult mouse tissues. Equal amounts (20 μg) of total protein lysates obtained from different CNS regions, as well as from several non-neural tissues of adult (48-week-old) mice, were subjected to standard immunoblotting (IB) procedure with the KCC2a antibody. A product of ∼140 kDa was detected in all CNS but not in non-neural tissues. Similar results were obtained in two independent experiments.
