FIGURE 3.

FRAP of aminoacyl-tRNA synthetases in the cytoplasm of living cell. FRAP was conducted on stably transformed cell lines. The areas of the bleach region where fluorescence recovery is monitored and of the distal region where fluorescence intensity is recorded in parallel are indicated with a closed and open symbols, respectively. The inset in A illustrates an experiment conducted with MetRS-GFP. Kinetics of fluorescence intensity variations in the bleach and distal regions are indicated with closed and open symbols, respectively. The experimental data were fitted to monoexponential curves to determine the half-times of fluorescence recovery. A, FRAP of MetRS-GFP and of GFP-ΔNMetRSΔC. B, FRAP of AsnRS-GFP and of ΔNAsnRS-GFP.