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Relative biosynthesis of 8R-HODE by 10R-DOX and 7,8-LDS. A, biosynthesis by recombinant 10R-DOX, L384M, L384V, and L384A. B, biosynthesis by recombinant 10R-DOX and by V388L and V388F. C, biosynthesis by recombinant 7,8-LDS and by V330L. The amino acids at studied positions are inserted over the bars. Untransfected insect cells transformed 18:2n-6 to negligible amounts of HODE (data not shown).
