TABLE 1.

Identification of protrudin-associated proteins by proteomics analysis

HEK293T cells transiently expressing 3× FLAG-tagged human protrudin were subjected to immunoaffinity chromatography with anti-FLAG. Proteins in the column eluate were digested with Lys-C endoproteinase, and the resulting peptide fragments were analyzed directly with a highly sensitive liquid chromatography-MS/MS system. The proteins reproducibly detected in two independent experiments are listed. The percentage of sequence coverage for each protein is also shown.

Protein	Sequence coverage
	%
Vesicle associated membrane protein-associated protein A (VAP-A)	14.5
Vesicle associated membrane protein-associated protein B and C (VAP-B/C)	14.4
T-complex protein 1, δ subunit (TCPD)	9.2
T-complex protein 1, γ subunit (TCPG)	7.7
T-complex protein 1, ε subunit (TCPE)	6.3