FIGURE 1.

FA localization of talin domain constructs. A, ligand binding sites for F-actin (ABS), β-integrins (IBS), vinculin (VBS), and the dimerization site (DS) of talin are shown. Constructs are indicated (bars), numbers refer to amino acid residues of the N/C terminus, and the relative strength of FA targeting is summarized (strong, weak, or none). Constructs used by others (19, 28), which we have re-analyzed for the purposes of comparison, are indicated (light gray bars). B, representative fluorescence images of GFP-tagged talin variants co-stained for vinculin to visualize FAs (red). Construct information is given as α-helices number (H) of the talin rod. Insets showing enlarged views of vinculin (v, top) or talin variants (t, middle) in FAs and the merged image (m, bottom) refer to selected regions (frames). Size bar, 20 μm (inset, 8 μm).