FA localization of talin domain constructs. A, ligand
binding sites for F-actin (ABS), β-integrins (IBS), vinculin
(VBS), and the dimerization site (DS) of talin are shown.
Constructs are indicated (bars), numbers refer to amino acid residues
of the N/C terminus, and the relative strength of FA targeting is summarized
(strong, weak, or none). Constructs used by others
(19,
28), which we have re-analyzed
for the purposes of comparison, are indicated (light gray bars).
B, representative fluorescence images of GFP-tagged talin variants
co-stained for vinculin to visualize FAs (red). Construct information
is given as α-helices number (H) of the talin rod.
Insets showing enlarged views of vinculin (v, top) or talin
variants (t, middle) in FAs and the merged image (m, bottom)
refer to selected regions (frames). Size bar, 20 μm
(inset, 8 μm).