FIGURE 1.

Schematic presentation and localization of INrf2 deletion domains. A, schematic presentation of INrf2 domains and deletion mutants. B, the INrf2 and deletion mutants were in vitro transcribed, translated, and analyzed by immunoblotting with anti-GFP antibodies. C, Hepa-1 cells were transfected with 0.5 μg of empty vector (1XGFP) or INrf2 or deletion mutants of INrf2. Whole cell lysate (W), cytoplasmic (C), and nuclear (N) protein fractions were prepared and analyzed by immunoblotting and probing with anti-GFP, anti-LDH, and anti-lamin B antibodies. D, Hepa-1 cells transfected with INrf2-1XGFP, INrf2ΔDGR-1XGFP, and DGR-2XGFP on slides were fixed in 2% formaldehyde, washed with PBS, stained with Vectashield containing nuclear DAPI stain, and mounted. Cells were observed under Nikon fluorescence microscope, and photographs were captured. The cytoplasmic and nuclear GFP fluorescence were quantified from 10 different transfected cells (n = 10) by using NIS-Elements BR2.30 SP4 software. All the experiments were repeated three times. Error bars indicate S.E. of fluorescence intensities.