FIGURE 5.

Ikaros degradation is mediated by the ubiquitin/proteasome pathway. A-D, whole cell lysates were prepared from 293T cells, as a negative control, or as indicated from untreated MOLT-4 or VL3-3M2 cells or MOLT-4 or VL3-M2 cells that had been treated with the MG132 proteasome inhibitor (MG). Whole cell lysates were immunoprecipitated (IP) with the indicated antibodies, and immunoprecipitates were analyzed by 9% SDS-PAGE followed by immunoblot (IB) with the indicated antibodies. The high molecular weight complexes containing Ikaros (IK)-ubiquitin (Ub) conjugates are marked. The Ikaros detected in MOLT-4 and VL3-3M2 cells in all panels is endogenous Ikaros.