TABLE 3.

Apparent molecular masses of LGP2 CTD complexes with different forms of dsRNA determined by gel filtration chromatography

Proteins, RNA, and protein/RNA complexes	Elution volume	Apparent molecular mass	Calculated molecular mass
	ml	kDa	kDa
LGP2 CTD	17.36	16.73	16.830
8-bp dsRNA	17.98	12.52	5.078
Small hairpin RNA	17.13	18.63	7.716
24-bp dsRNA	16.08	30.43	15.426
24-bp 5′ ppp dsRNA	15.70	36.31	15.666
5′ overhang 22-bp dsRNA	16.18	29.04	14.171
3′ overhang 22-bp dsRNA	15.86	33.73	14.171
LGP2 CTD and 8-bp dsRNA	15.74	35.67	21.908 (1:1), 38.738 (2:1)a
LGP2 CTD and small hairpin dsRNA	15.97	32.03	24.546 (1:1), 41.376 (2:1)
LGP2 CTD and 24-bp dsRNA	14.40	66.72	32.256 (1:1), 49.086 (2:1)
LGP2 CTD and 24-bp 5′ ppp dsRNA	14.78	55.85	32.496 (1:1), 49.326 (2:1)
LGP2 CTD and 5′ overhang 22-bp dsRNA	15.04	49.48	31.001 (1:1), 47.831 (2:1)
LGP2 CTD and 3′ overhang 22-bp dsRNA	15.70	36.34	31.001 (1:1), 47.831 (2:1)

^aCalculated molecular masses of the complexes with 1:1 and 2:1 stoichiometry.