FIGURE 4.

Unfolding force versus stretch speed in absence ofαB crystallin (control) and in the presence of WT, R120G, and R157H αB-crystallin. At each stretch speed, we determined the mean and S.E. of all force peaks. Stretch speed was corrected for cantilever bending (see “Experimental Procedures” for details). Results were fit with linear lines (control, y = -89 + 89x; WT αB-crystallin, y = -72 + 101x; R120G, y = -129 + 104x; R157H, y = -27 + 78x). See “Experimental Procedures” for additional details).