TABLE 1.

Persistence length of N2B-Us (nm) as a function of pulling speed (nm/s) and the absence (control) or presence of αB-crystallin (wild type and R120G and R157H mutants)

Values in parenthesis were obtained at pH 6.4. All others were at pH 7.4. The number of molecules per group was ∼30-40.

Speed	Control	αB-crystallin
		Wild type	R120G	R157H
nm/s	nm		nm
500	0.71 ± 0.19	0.21 ± 0.04a	0.63 ± 0.05b	0.31 ± 0.02c,d,e
1000	0.70 ± 0.11	0.18 ± 0.02a	0.69 ± 0.14b	0.36 ± 0.02c,d,e
2000	0.65 ± 0.05	0.20 ± 0.03a	0.67 ± 0.16f	0.29 ± 0.02a,f,g
	(0.62 ± 0.12)	(0.17 ± 0.01)a
4000	0.67 ± 0.13	(0.18 ± 0.02)a

^ap < 0.001 compared with control.

^bp < 0.001 compared with wild type αB-crystallin.

^cp < 0.01 compared with control.

^dp < 0.05 compared with wild type αB-crystallin.

^ep < 0.01 compared with R120G.

^fp < 0.01 compared with wild type αB-crystallin.

^gp < 0.001 compared with R120G.