TABLE 4.
Average unfolding force (pN) of Ig domains in Ig 1-8 and Ig 8-15 at pulling speed 1000 nm/s in the absence (control) or presence of αB-crystallin (wild type and R120G and R157H mutants)
The average unfolding force of each of the eight unfolding peaks was compared in a paired t test. The number of molecules per group was ∼25-30.
|
Control
|
αB-crystallin
|
|||
|---|---|---|---|---|
| Wild type | R120G | R157H | ||
| pN | pN | |||
| Ig1-8 | 221 ± 11 | 254 ± 13a | 224 ± 14 NSb | 248 ± 12a,c |
| Ig8-15 | 232 ± 12 | 263 ± 16a | 223 ± 10 NSb | 257 ± 13a,c |
a
p < 0.001 compared with control.
b
p < 0.001 compared with wild type αB-crystallin.
c
p < 0.001 compared with R120G.