FIGURE 10.

Phosphoinositide metabolism at clathrin-coated buds. Schematic representation of key enzymes responsible for PtdIns(4,5)P₂ synthesis and the mapped interactions with the AP-2 adaptor appendages. The location of the two separate PtdIns(4,5)P₂ binding sites on the AP-2 heterotetramer is indicated with purple spots. DAG, diacylglycerol; PLD, phospholipase D; PLC, phospholipase C; PIK, phosphatidylinositol 4-kinase; PtdCho, phosphatidylcholine; Ins(1,4,5)P₃, inositol 1,4,5-trisphosphate; PtdIns, phosphatidylinositol; PtdIns(4)P, phosphatidylinositol 4-phosphate.