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. 1999 Mar 2;96(5):2396–2401. doi: 10.1073/pnas.96.5.2396

Figure 6.

Figure 6

Caspase-1 is essential for the induction of apoptosis. (A) Protection by YVAD of RAW264.7 macrophages from Salmonella-induced cell death. At 1 hr before infection with S. typhimurium SL1344 at an moi of either 100:1 (1 and 2) or 50:1 (3 and 4), cells were preincubated with 50 μM Ac-YVAD-CMK (2 and 4) or media alone (1 and 3). The presence of the caspase-1 inhibitor led to a decrease in cytotoxicity. (B) Caspase-1 is necessary for Salmonella-induced cytotoxicity. Peritoneal macrophages from either wild-type or caspase-1 −/− mice were infected for 8 hr with S. typhimurium SL1344 or BJ66. SL1344 killed the macrophages from the wild-type mice, whereas neither SL1344 nor BJ66 were able to kill macrophages lacking caspase-1. The means and SDs of three experiments are shown.