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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Gen Virol. 2008 Oct;89(Pt 10):2611–2621. doi: 10.1099/vir.0.2008/003624-0

Fig. 3.

Fig. 3

HIV-1 Env-mediated fusion with immortalized CCR5+/+ PBMC lines transduced with lentivirus vectors encoding either CCR5 or CCR5Δ32. PBMC lines transduced with the indicated lentivirus vectors were infected with a vaccinia virus recombinant encoding pT7-lacZ. The effect of the CCR5Δ32 protective effect in donor #1 (a), donor #2 (b) and donor #3 (c) was analysed by mixing the pT7-lacZ-expressing PBMC lines with HeLa cells expressing the indicated HIV-1 Env and T7 RNA polymerase and determining the amount of β-galactosidase resulting from cell fusion. VSV Env fusion was used as a positive control to indicate the competence of the PBMC lines in cell fusion (not shown). In all experiments the X4 Env was from isolate LAV and the R5 Env was from isolate Ba-L.