. Author manuscript; available in PMC: 2009 Oct 1.

Published in final edited form as: J Gen Virol. 2008 Oct;89(Pt 10):2611–2621. doi: 10.1099/vir.0.2008/003624-0

Table 1. Cell-surface expression of CCR5 and CXCR4 in CD4⁺ T lymphocytes isolated from immortalized PBMCs.

Results are shown as mean fluorescence values. Numbers in parentheses indicate the mean values obtained with cells stained with the matched isotype control. IMM, Immortalized

Receptor	Donor #1		Donor #2		Donor #3
Receptor	IMM	PHA+IL-2^*	IMM	PHA+IL-2^*	IMM	PHA+IL-2^*
CCR5	63 (2.6)	28 (2.5)	78 (2.7)	21.6 (3.2)	38 (3.4)	24 (3.9)
CXCR4	255 (2.6)	185 (3.7)	242 (3.5)	169 (4.6)	389 (3.9)	268 (3.7)

Unstimulated PBMCs from each donor were frozen in liquid nitrogen until the immortalized cells from the three donors were ready. Once the immortalized lines were established, the PBMC samples were thawed, stimulated with PHA and IL-2, and used to purify CD4⁺ T cells as described in Methods.

Table 1. Cell-surface expression of CCR5 and CXCR4 in CD4+ T lymphocytes isolated from immortalized PBMCs.

Table 1. Cell-surface expression of CCR5 and CXCR4 in CD4⁺ T lymphocytes isolated from immortalized PBMCs.