. Author manuscript; available in PMC: 2009 Oct 1.

Published in final edited form as: J Gen Virol. 2008 Oct;89(Pt 10):2611–2621. doi: 10.1099/vir.0.2008/003624-0

Table 2. Cell-surface expression of CCR5 and CXCR4 in CD4⁺ T lymphocytes isolated from lentivirus-transduced PBMC lines.

Transduced/immortalized PBMCs from the three donors were cultured for 6 months in the presence of blasticidin S and then used to purify CD4⁺ T lymphocytes. The CD4⁺ T cells were analysed for receptor/co-receptor cell-surface expression. Results are shown as mean fluorescence values. Numbers in parentheses indicate the mean values obtained with cells stained with the matched isotype control

Receptor	Transduced lentivirus
	Donor #1			Donor #2			Donor #3
	Vector	CCR5	CCR5Δ32	Vector	CCR5	CCR5Δ32	Vector	CCR5	CCR5Δ32
CCR5	78 (1.8)	142 (2.1)	27 (2.3)	110 (2.6)	176 (2.3)	34 (2.2)	74 (3.8)	178 (2.1)	63 (2.2)
CXCR4	225 (1.9)	252 (2.2)	75 (2.2)	222 (3.1)	232 (2.7)	81 (3.5)	419 (3.9)	435 (3.4)	387 (3.8)

Table 2. Cell-surface expression of CCR5 and CXCR4 in CD4+ T lymphocytes isolated from lentivirus-transduced PBMC lines.

Table 2. Cell-surface expression of CCR5 and CXCR4 in CD4⁺ T lymphocytes isolated from lentivirus-transduced PBMC lines.