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. 2008 Dec 17;117(4):611–616. doi: 10.1289/ehp.0800235

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Detection of free radicals from DEP (10 μg/mL) using EPR. (A) Sample spectra after 60 min showing the characteristic three-peaked spectrum produced by oxidation of the spin trap Tempone-H (1 mM); DEP (10 μg/mL) caused a large increase in peak amplitude, an effect that was partially reduced by coincubation with SOD (100 U/mL). (B) EPR intensity generated by auto-oxidation (control), DEP, or pyrogallol (100 μM) over a 1-hr period, in the presence (open symbols) or absence of SOD (solid symbols). EPR intensity is calculated using the area under the curve of the first derivative of the first line of the three-line spectrum generated. Data are mean ± SE (n = 6).

Paired t-tests: *p < 0.05, DEP versus DEP + SOD; **p < 0.01, control versus DEP; ^††p < 0.01, pyrogallol versus pyrogallol + SOD.