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. Author manuscript; available in PMC: 2009 May 8.
Published in final edited form as: Tumour Biol. 2008 Jul 9;29(3):161–180. doi: 10.1159/000143402

Figure 1. Immunoelectrophoretic identification of AFP.

Figure 1

To produce antiserum to AFP rabbits were immunized with rat amniotic fluid (AMF) and the anti-AMF serum was absorbed with normal adult rat serum (NARS). Later AFP was isolated by isoelectric focusing and a specific antiserum was obtained by immunization of a goat with purified AFP (anti-α1-F). CARS- Croton oil induced acute phase rat serum (contains α1- macrofetoglobulin, αM-F); abs – absorbed; NARS-normal adult rat serum; TR-transferrin; ALB-albumin; γG-gamma-globulin; FRS-fetal rat serum. Modified from Sell, et al. [15]