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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Immunol. 2008 Nov 2;9(12):1388–1398. doi: 10.1038/ni.1667

Figure 2. Foxo1 regulates IL-7Rα expression and pro-B cell survival.

Figure 2

(a) IL-7Rα expression on pro-B cells from Foxo1+/+mb1Cre (red) and Foxo1L/Lmb1Cre (blue) mice. (b) Ann V expression on late-pro-B cells from Foxo1+/+mb1Cre (red) and Foxo1L/Lmb1Cre (blue) mice. (c) Intracellular Bim expression in pro-B (line) and non-B (shaded) cells and MFI analysis of Bim expression from pro-B and non-B cells (5 mice of each group indicated with symbols, and average MFI indicated with dashes). (d) Intracellular Bcl-xL expression of pro-B (line) and non-B (shaded) cells and MFI of Bcl-xL expression of pro-B and non-B cells (3–4 mice of each group). (e) Foxo1+/+ and Foxo1L/L B220+ bone marrow cells were grown in culture with IL-7 for 2 days, infected with MSCV-Bcl-xL or left uninfected, followed by infection with MIT or MIT-Cre. MIT-Cre-transduced (Thy1.1+) B cells were enumerated by FACS on day 2 and day 5. (f) Bcl-xL infected cells superinfected with either MIT or MIT-Cre were labeled with CFSE and cultured for 4 days. FACS analysis of proliferation was determined by CFSE division. FACS profiles are representative of 3–5 independent experiments.