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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Immunol. 2008 Nov 2;9(12):1388–1398. doi: 10.1038/ni.1667

Figure 8. Foxo1 regulates AID expression.

Figure 8

(a) Flow cytometric analysis of LPS + IL-4 stimulated (3 days) B cells from Foxo1+/+Cd21Cre and Foxo1L/LCd21Cre mice (2 mice per group repeated in 3 independent experiments). Proliferation was measured by CFSE partitioning on plasmablasts (Syn1+) undergoing CSR (IgG+). (b) Percentage of class switched cells generated in the absence or presence of the PI(3)Kδ inhibitor IC87114 (+IC8). Data is representative of 3 independent experiments. (c) Representative semi-quantitative PCR from two independent experiments to measure Aicda expression and the generation of γ1 germline transcripts (GLT), γ1 post-switch transcripts (PST), and γ1 circular transcripts (CT-lower band). (d) QT-PCR of Aicda expression of splenic germinal center B cells from Foxo1+/+Cd21Cre, Foxo3a−/−, and Foxo1L/LCd21Cre mice (3 mice per group) 10 days after immunization with 200 μg of NP-KLH in alum.