Figure 6.

MEF2CA-ESC-derived neuronal progenitor cells injected into mouse stroke model express neuronal markers and migrate into ischemic areas of the brain. A, B, Four weeks after transplantation, MEF2CA/EGFP-ESC-derived NPCs (B) but not EGFP (control)-expressing progenitors (A) that were located near the injection site stained for the early neuronal marker TuJ1. Cells were colabeled with BrdU before transplantation for identification purposes. Scale bar, 25 μm. C, D, By 8 weeks after transplantation, many of the injected MEF2CA/EGFP-ESC-derived NPCs had migrated to an area adjacent to the ischemic zone and expressed the mature neuronal marker NeuN (C). Scale bar, 200 μm. Colocalization of EGFP and NeuN can be seen more clearly in the high-power inset (D). Scale bar, 15 μm.