Table 4.
rhArg1 (U/ml) | rhArg1-peg5,000 mw (U/ml) | |
HepG2 | 0.18 ± 0.06 | 0.22 ± 0.03 |
Hep3B | 0.07 ± 0.03 | 0.10 ± 0.04 |
HepG2 and Hep3B were incubated in arginine-containing DMEM medium supplemented with 10% FBS with rhArg or rhArg1-peg5,000 mw (0–100 U/ml) for 72 h at 37°C/5% CO2. IC50 values were calculated as the concentration of native rhArg1 or pegylated rhArg1 which inhibited 50% cell growth. The experiments were repeated three times.