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Cell surface expression of membrane-bound immunomodulator fusion constructs. Cell surface immunofluorescent staining of wild-type MDCK cells (A, B, C) and MDCK CYT-IVAC producer cells expressing membrane-bound mouse GM-CSF/HA (D), IL-2/HA (E), or IL-4/HA (F). Paraformaldehyde fixed cells were labeled using rat anti-GM-CSF (A, D), anti-IL2 (B, E) or anti-IL4 (C, F) specific antibodies followed by Alexa Flour® 488 conjugated secondary antibody.
