Table 1.
Characterization of CYT-IVAC hemagglutinin and cytokine content
| Vaccine | HA pixel density* | HAU/μg of vaccine |
Total cytokine (ng/μg vaccine)** |
Bioactive cytokine (pg/μg vaccine)*** |
| PR/8/34 w.t. | 5835.4 | 16 | NA | NA |
| PR/8/34 GM-CSF/HA | 6407.9 | 16 | 185 ± 21 | 87.3 |
| PR/8/34 IL-2/HA | 5562.9 | 32 | 4.92 ± 0.3 | 411 |
| PR/8/34 IL-4/HA | 6090.4 | 32 | 176 ± 24 | 456 |
* Pixel density of HA specific chemiluminescent signal following equal loading of total viral protein
** Quantitation of virus-incorporated cytokine on protein level based on standard curve of recombinant cytokine (ng of cytokine per ug of vaccine)
*** Quantitation of virus-incorporated cytokine on bioactive level based on standard curve of recombinant cytokine (pg of cytokine per ug of vaccine)