G2A deficiency increases ApoA1 and ApoE in plasma HDL of LDLR-/- mice. A, Online cholesterol analysis of plasma lipoproteins in high-cholesterol diet-fed (20 weeks) male LDLR-/-G2A+/+ and LDLR-/-G2A-/- mice by FPLC (CLIP). Data shown for individual mice is representative of 3 independent experiments. Inset, Anti-ApoA1 immunoblot of plasma from the same mice. B, FPLC separation of plasma lipoproteins from high-cholesterol diet-fed (20 weeks) LDLR-/-G2A+/+ and LDLR-/-G2A-/- mice. Inset, Immunoblot of pooled consecutive pairs of HDL fractions (indicated by dashed lines) with antibodies specific for ApoA1 and ApoE (1: LDLR-/-G2A+/+, 2: LDLR-/-G2A-/-). C, Densitometric quantification of ApoA1 and ApoE immunoreactivity in the indicated HDL fractions from B.