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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Exp Neurol. 2009 Feb 10;217(1):124–135. doi: 10.1016/j.expneurol.2009.01.022

Figure 2.

Figure 2

DeOlmos cupric silver degeneration staining in PPT1−/− mice. (A–D) Representative coronal images of silver staining in the anterior cerebellum of WT (A), 6 mo. PPT1−/− (B), 7 mo. PPT1−/− (C), and 8 mo. PPT1−/− mice (D). Notice the overall increase in silver staining as the mice age, most notably within the cerebellar white matter. (E) Higher magnification image of the 7 mo. PPT1−/− cerebellum. In addition to the cerebellar white matter, silver stained parasagittal bands, or blackened stripes, are visualized in the molecular layer in the cerebellar cortex. Locator boxes identify the areas magnified in subsequent panels. (F & G) High magnification of parasagittal banding found in an individual folia of PPT1−/− mice at 7 mo. The banding seen in the molecular layer is staining of the dendritic arbors of diseased Purkinje cells. Blackened debris litters the Purkinje cell layer, most likely due to dying Purkinje cells. (H) High magnification of a degenerating Purkinje cell, with a blackened soma and dendritic arbor. (I) High magnification image of the cerebellar white matter tracts contains degenerating efferent and afferent projections to the cerebellar cortex. Degenerating axons represent the sole output of the PPT1−/− cerebellum.